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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Stable and selective permeable hydrogel microcapsules for high-throughput cell cultivation and enzymatic analysis

Fig. 2

Polyelectrolyte Coating modifies Microcapsule Permeability. HC-CAMs uncoated and coated with PSS/PAH were loaded either with FITC-peptide or with GFP. The release of the two molecules from HC-CAMs and HC-PCAMs was analyzed upon incubation in a Tris buffer solution. a HC-CAMs loaded with either FITC-peptide or GFP exhibited no fluorescence after 10 min’ incubation when analyzed by fluorescence microscopy. The scale bar corresponds to 200 µm. b HC-PCAMs loaded with FITC-peptide exhibited no fluorescence after 24 h’ incubation, while those loaded with GFP exhibited a strong fluorescence signal, showing that HC-PCAMs are permeable to FITC-peptide but efficiently retain GFP (left panel). Kinetic release profiles of FITC-peptide (black circles) and GFP (open circles) in the Tris buffer solution from FITC-peptide and GFP-loaded HC-PCAMs respectively. Samples of buffer were taken at different time points (0, 0.5, 1, 2, 4, 8, 24 h) and fluorescence was measured using a plate reader. Data were plotted against time and fitted using the exponential decay equation (right panel) n = 2

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