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Table 1 Total protein yield (μg) of each recombinant construct at different times post-induction (1, 3, and 5 h)

From: Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications

 

GFP

J-GFP-F

J/F-GFP

p value

Time (h)

1

3

5

1

3

5

1

3

5

Treat

Time

Treat × time

Protein yield (μg)

52.39 ± 0.79

99.43 ± 1.03

223.82 ± 1.48

1.44 ± 0.12

1.23 ± 0.08

0.93 ± 0.07

5.12 ± 0.23

9.98±0.2

20.66 ± 1.96

< 0.0001

0.029

0.014