Table 1 DNA sequence of PCR primers used for his6-phoA gene cloning and engineering
Primer name | DNA sequence | Target |
|---|---|---|
FAlkPHisBsaI | 5′-CCCCGGTCTCTCATGCCAATGTCTCACCACCA TCACCACCATAGAACACCAGAAATGCCTGT-3′ | wt phoA gene GenBank M29663.1 protein id AAA24363.1 |
RAlkPHindIII | 5′-CACGCCGGGCAAGCTTTTATTTCAGCC-3′ | wt phoA gene GenBank M29663.1 protein id AAA24363.1 |