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Table 3 Determination of specific activities of MDH, NAPRTase, PPC, PYC and PCK in crude extracts of the control and the recombinant strains

From: Metabolic engineering of Escherichia coli for l-malate production anaerobically

Strain MDH activity (U/mg) NAPRTase activity (U/mg) PPC activity (U/mg) PYC activity (U/mg) PCK activity (U/mg)
BA040 3.80 ± 0.07 1.94 ± 0.04 2.24 ± 0.12 NDa 0.03 ± 0.01
BA041 72.80 ± 0.06 2.05 ± 0.05 2.26 ± 0.05 ND 0.01 ± 0.01
BA042 4.80 ± 0.08 19.45 ± 0.25 3.11 ± 0.05 ND 0.02 ± 0.01
BA050 3.50 ± 0.07 0.10 ± 0.01 2.43 ± 0.04 ND 0.02 ± 0.01
BA051 3.63 ± 0.03 1.49 ± 0.02 20.03 ± 0.23 ND 0.01 ± 0.01
BA052 4.50 ± 0.05 1.47 ± 0.03 3.02 ± 0.15 1.82 ± 0.14 0.01 ± 0.01
BA061 5.20 ± 0.05 1.50 ± 0.02 ND ND 1.94 ± 0.06
BA062 4.80 ± 0.12 18.74 ± 0.35 ND ND 2.15 ± 0.05
BA063 71.70 ± 2.50 1.80 ± 0.04 ND ND 2.24 ± 0.08
  1. Each value is the mean of three parallel replicates ± standard deviation
  2. aND, not detected