Table 2 Strains and plasmids used in this study
Strain or plasmid | Characteristic(s) | References or sources |
|---|---|---|
Strain | ||
Escherichia coli S17-1 | recA, pro, thi, conjugative strain able to host λ-pir-dependent plasmids | [43] |
Enterobacter cloacae SDM | Wild-type | [12] |
E. coli BL21-pETRABC | E. coli BL21 (DE3) harboring pET-RABC | [26] |
Klebsiella pneumonia ATCC 15380 | Wild-type | ATCC |
Bacillus licheniformis DSM13 | Wild-type | DSMZ |
Klebsiella oxytoca PDL-0 | Wild-type | CCTCC M 2016184 |
K. oxytoca PDL-K1 | K. oxytoca PDL-0 with deletion of pox | This study |
K. oxytoca PDL-K2 | K. oxytoca PDL-0 with deletion of pox and pta | This study |
K. oxytoca PDL-K3 | K. oxytoca PDL-0 with deletion of pox, pta, and frdA | This study |
K. oxytoca PDL-K4 | K. oxytoca PDL-0 with deletion of pox, pta, frdA, and ldhD | This study |
K. oxytoca PDL-K5 | K. oxytoca PDL-0 with deletion of pox, pta, frdA, ldhD, and pflB | This study |
Plasmid | ||
pKR6KCm | Cmr, gene replacement vector derived from plasmid pK18mobsacB, R6K origin, Mob+ sacB, and the Kmr resistance was replaced by Cmr | [27] |
pKDΔpox | pKR6KCm derivative, carries a 580 bp deletion of pox | This study |
pKDΔpta | pKR6KCm derivative, carries a 1152 bp deletion of pta | This study |
pKDΔfrdA | pKR6KCm derivative, carries a 720 bp deletion of frdA | This study |
pKDΔldhD | pKR6KCm derivative, carries a 386 bp deletion of ldhD | This study |
pKDΔpflB | pKR6KCm derivative, carries a 1150 bp deletion of pflB | This study |