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Table 2 Strains and plasmids used in this study

From: Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Strain or plasmid Characteristic(s) References or sources
Strain
 Escherichia coli S17-1 recA, pro, thi, conjugative strain able to host λ-pir-dependent plasmids [43]
 Enterobacter cloacae SDM Wild-type [12]
 E. coli BL21-pETRABC E. coli BL21 (DE3) harboring pET-RABC [26]
 Klebsiella pneumonia ATCC 15380 Wild-type ATCC
 Bacillus licheniformis DSM13 Wild-type DSMZ
 Klebsiella oxytoca PDL-0 Wild-type CCTCC M 2016184
 K. oxytoca PDL-K1 K. oxytoca PDL-0 with deletion of pox This study
 K. oxytoca PDL-K2 K. oxytoca PDL-0 with deletion of pox and pta This study
 K. oxytoca PDL-K3 K. oxytoca PDL-0 with deletion of pox, pta, and frdA This study
 K. oxytoca PDL-K4 K. oxytoca PDL-0 with deletion of pox, pta, frdA, and ldhD This study
 K. oxytoca PDL-K5 K. oxytoca PDL-0 with deletion of pox, pta, frdA, ldhD, and pflB This study
Plasmid
 pKR6KCm Cmr, gene replacement vector derived from plasmid pK18mobsacB, R6K origin, Mob+ sacB, and the Kmr resistance was replaced by Cmr [27]
 pKDΔpox pKR6KCm derivative, carries a 580 bp deletion of pox This study
 pKDΔpta pKR6KCm derivative, carries a 1152 bp deletion of pta This study
 pKDΔfrdA pKR6KCm derivative, carries a 720 bp deletion of frdA This study
 pKDΔldhD pKR6KCm derivative, carries a 386 bp deletion of ldhD This study
 pKDΔpflB pKR6KCm derivative, carries a 1150 bp deletion of pflB This study