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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Fig. 2

Selection for stains that can produce BDO from lactose. Biomass (a), consumption of lactose (b), concentration (c) and yield (d) of BDO using lactose as the carbon source by E. cloacae SDM, E. coli BL21-pETRABC, B. licheniformis DSM13, K. pneumonia ATCC 15380, and K. oxytoca PDL-0 were assayed. The experiments were conducted in a 300-mL flask containing 50 mL of M9 minimal medium supplemented with 5 g/L yeast extract and 40 g/L lactose with shaking at 180 rpm for 48 h. The culture temperature for B. licheniformis DSM13 was 50 °C while for other strains were 37 °C. The data for K. oxytoca PDL-0 and K. pneumoniae ATCC 15380 were obtained at 18 h and 36 h, respectively. The data for E. cloacae SDM, B. licheniformis DSM13 and E. coli BL21-pETRABC were obtained at 48 h. Error bars indicate the standard deviations from three independent cultures

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