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Table 2 Effects of initial substrate concentration in the aqueous phase on the biosynthesis of cinnamyl alcohol in the biphasic system

From: Efficient biosynthesis of cinnamyl alcohol by engineered Escherichia coli overexpressing carboxylic acid reductase in a biphasic system

The initial substrate in the aqueous phase (mM)

Organic phase (mM)

Aqueous phase (mM)

Yield (%)

Conversion (%)

Cinnamyl alcohol

Cinnamaldehyde

Cinnamyl alcohol

Cinnamaldehyde

17.4

37.36 ± 0.24

4.34 ± 0.19

0.41 ± 0.06

0.13 ± 0

88.2 ± 0.56

100 ± 0.05

24.7

34.13 ± 0

22.84 ± 0

0.52 ± 0

0.21 ± 0

59.0 ± 0

100 ± 0.04

32.0

27.44 ± 0

31.40 ± 0

0.27 ± 0

0.22 ± 0

45.5 ± 0

77.2 ± 0.03

  1. Conditions: 3 mL of 100 mM phosphate buffer (pH 7.5) consisting of 17.432 mM cinnamic acid, 102 mM glucose, wet cells (OD600nm 50) and 1.2 mL of dibutyl phthalate, 200 rpm, 30 °C; the reaction lasted 6 h under the above conditions