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Table 2 Effects of initial substrate concentration in the aqueous phase on the biosynthesis of cinnamyl alcohol in the biphasic system

From: Efficient biosynthesis of cinnamyl alcohol by engineered Escherichia coli overexpressing carboxylic acid reductase in a biphasic system

The initial substrate in the aqueous phase (mM) Organic phase (mM) Aqueous phase (mM) Yield (%) Conversion (%)
Cinnamyl alcohol Cinnamaldehyde Cinnamyl alcohol Cinnamaldehyde
17.4 37.36 ± 0.24 4.34 ± 0.19 0.41 ± 0.06 0.13 ± 0 88.2 ± 0.56 100 ± 0.05
24.7 34.13 ± 0 22.84 ± 0 0.52 ± 0 0.21 ± 0 59.0 ± 0 100 ± 0.04
32.0 27.44 ± 0 31.40 ± 0 0.27 ± 0 0.22 ± 0 45.5 ± 0 77.2 ± 0.03
  1. Conditions: 3 mL of 100 mM phosphate buffer (pH 7.5) consisting of 17.432 mM cinnamic acid, 102 mM glucose, wet cells (OD600nm 50) and 1.2 mL of dibutyl phthalate, 200 rpm, 30 °C; the reaction lasted 6 h under the above conditions