Fig. 6

Time-course profiles of cinnamyl alcohol biosynthesis from l-phenylalanine. Conditions: 3 mL of 100 mM phosphate buffer (pH 8.5) consisting of 40 mM l-phenylalanine, E. coli BLP3 (OD_600nm 15), 200 rpm, 30 °C; the reaction lasted 2 h under the above conditions to synthesize cinnamic acid. The reaction mixture was spun down, after which the 1.5 mL of the supernatant was supplemented with 102 mM glucose and E. coli BLCS (OD_{600 nm} 50) until the volume of the aqueous phase reached 3 mL. The second reaction was started after the addition of 1.2 mL of dibutyl phthalate and was carried out at 30 °C and 200 rpm for 6 h