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Table 1 Strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor

Strains Description Reference
XL-1 Blue Cloning strain Stratagene
BW25113 E. coli ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), \(\lambda\)-, rph-1, ∆(rhaD- rhaB)568, hsdR514 Invitrogen
JW2670-1 BW25113 ΔpncC::kan Yale E. coli Genetic Stock Center
MX101 BW25113 ΔpncC ΔnadR::kan [13]
72c E. coli F-, lacZ4, nadD72(ts,Fs), \(\lambda\), argG75 [28]
Plasmids Descriptions Reference
pWB203 PLlacO1::Ft nadE - Ft nadV, ColE1 ori, AmpR [13]
pWB301 PLlacO1::Ec yqhD, ColE1 ori, AmpR This study
pWB302 PLlacO1::Se pnuC* KA, ColE1 ori, AmpR This study
pWB303 PLlacO1::Ft nadE, ColE1 ori, AmpR This study
pWB304 PLlacO1::Ec pnuC - Se nadR, ColE1 ori, AmpR This study
pWB305 PLlacO1::Ec pnuC - Sc NRK1, ColE1 ori, AmpR This study
pWB306 PLlacO1::Hd nadV, ColE1 ori, AmpR This study
pWB307 PLlacO1::Rs nadV, ColE1 ori, AmpR This study
pWB308 PLlacO1:: Ss nadV, ColE1 ori, AmpR This study
pWB309 PLlacO1:: SynE nadV, ColE1 ori, AmpR This study
pDB102 PLlacO1::Ft nadE - Rs nadV, ColE1 ori, AmpR This study
pDB103 PLlacO1::Ft nadE - Ss nadV, ColE1 ori, AmpR This study
pDB104 PLlacO1::Ft nadE - SynE nadV, ColE1 ori, AmpR This study
pLZ301 PLlacO1::Ft nadE - Hd nadV, ColE1 ori, AmpR This study
  1. Abbreviations indicate source of genes: Ec Escherichia coli, Se Salmonella enterica, Ft Francisella tularensis, Sc Saccharomyces cerevisiae, Rs Ralstonia solanacearum, Ss Synechocystis sp. PCC 6803, SynE Synechococcus elongatus PCC 7942, Hd Haemophilus ducreyi. Se pnuC* KA contains mutations compared to the wild type sequence (see “Methods” and Additional file 1)