Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor

Table 1 Strains and plasmids used in this study

Strains	Description	Reference
XL-1 Blue	Cloning strain	Stratagene
BW25113	E. coli ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), \(\lambda\)^-, rph-1, ∆(rhaD- rhaB)568, hsdR514	Invitrogen
JW2670-1	BW25113 ΔpncC::kan	Yale E. coli Genetic Stock Center
MX101	BW25113 ΔpncC ΔnadR::kan	[13]
72c	E. coli F-, lacZ4, nadD72(ts,Fs), \(\lambda\)⁻, argG75	[28]

Plasmids	Descriptions	Reference
pWB203	P_LlacO1::Ft nadE - Ft nadV, ColE1 ori, Amp^R	[13]
pWB301	P_LlacO1::Ec yqhD, ColE1 ori, Amp^R	This study
pWB302	P_LlacO1::Se pnuC* KA, ColE1 ori, Amp^R	This study
pWB303	P_LlacO1::Ft nadE, ColE1 ori, Amp^R	This study
pWB304	P_LlacO1::Ec pnuC - Se nadR, ColE1 ori, Amp^R	This study
pWB305	P_LlacO1::Ec pnuC - Sc NRK1, ColE1 ori, Amp^R	This study
pWB306	P_LlacO1::Hd nadV, ColE1 ori, Amp^R	This study
pWB307	P_LlacO1::Rs nadV, ColE1 ori, Amp^R	This study
pWB308	P_LlacO1:: Ss nadV, ColE1 ori, Amp^R	This study
pWB309	P_LlacO1:: SynE nadV, ColE1 ori, Amp^R	This study
pDB102	P_LlacO1::Ft nadE - Rs nadV, ColE1 ori, Amp^R	This study
pDB103	P_LlacO1::Ft nadE - Ss nadV, ColE1 ori, Amp^R	This study
pDB104	P_LlacO1::Ft nadE - SynE nadV, ColE1 ori, Amp^R	This study
pLZ301	P_LlacO1::Ft nadE - Hd nadV, ColE1 ori, Amp^R	This study

Abbreviations indicate source of genes: Ec Escherichia coli, Se Salmonella enterica, Ft Francisella tularensis, Sc Saccharomyces cerevisiae, Rs Ralstonia solanacearum, Ss Synechocystis sp. PCC 6803, SynE Synechococcus elongatus PCC 7942, Hd Haemophilus ducreyi. Se pnuC* KA contains mutations compared to the wild type sequence (see “Methods” and Additional file 1)

ISSN: 1475-2859