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Fig. 1 | Microbial Cell Factories

Fig. 1

From: A common approach for absolute quantification of short chain CoA thioesters in prokaryotic and eukaryotic microbes

Fig. 1

LC–MS/MS chromatogram of a synthetic CoA thioester standard sorted by the retention time of each analyte (a). LC–MS/MS chromatogram of C. glutamicum LYS-9 (b, c). Co-eluting CoA thioesters were distinguished by their different specific mass-to-charge ratio (m/z), except for butyryl- and isobutyryl-CoA. Analysis was performed on a triple quadrupole MS (AB Sciex QTRAP 6500+, AB Sciex Germany GmbH, Darmstadt, Germany) coupled to an HPLC system (Agilent Infinity 1290 System)

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