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Table 2 Mucic acid production from poly-d-galacturonic acid by marine Trichoderma sp. LF328 transformants

From: Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

Strain

T2

T3

D-161646

Lactose + poly-d-galacturonate

 Mucic acid (g L−1)

3.6 ± 0.1a

2.9 ± 0.6a

1.7 ± 0.0b

 Conversion mucic acid/available d-galacturonic acid (g g−1)

0.42 ± 0.01

0.34 ± 0.07

0.20 ± 0.01

d-Xylose + poly-d-galacturonate

 Mucic acid (g L−1)

3.6a

3.6 ± 0.2a

0.1b

 Conversion mucic acid/available d-galacturonic acid (g g−1)

0.40

0.39

0.01

  1. Trichoderma sp. LF328 transformants were grown in 24-well plates using poly-d-galacturonic acid as substrate and either lactose or d-xylose as co-substrate. T. reesei Δgar1 udh strain D-161646 was included as a positive control. Values are mean ± standard error of the mean for n = 2 (D-161646) or 3 biological replicates. Values for mucic acid concentration in the same row with the same superscript letter (a or b) did not differ significantly (p > 0.05)