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Table 2 Mucic acid production from poly-d-galacturonic acid by marine Trichoderma sp. LF328 transformants

From: Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

Strain T2 T3 D-161646
Lactose + poly-d-galacturonate
 Mucic acid (g L−1) 3.6 ± 0.1a 2.9 ± 0.6a 1.7 ± 0.0b
 Conversion mucic acid/available d-galacturonic acid (g g−1) 0.42 ± 0.01 0.34 ± 0.07 0.20 ± 0.01
d-Xylose + poly-d-galacturonate
 Mucic acid (g L−1) 3.6a 3.6 ± 0.2a 0.1b
 Conversion mucic acid/available d-galacturonic acid (g g−1) 0.40 0.39 0.01
  1. Trichoderma sp. LF328 transformants were grown in 24-well plates using poly-d-galacturonic acid as substrate and either lactose or d-xylose as co-substrate. T. reesei Δgar1 udh strain D-161646 was included as a positive control. Values are mean ± standard error of the mean for n = 2 (D-161646) or 3 biological replicates. Values for mucic acid concentration in the same row with the same superscript letter (a or b) did not differ significantly (p > 0.05)