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Table 1 Mucic acid production characteristics of marine fungi in 24-well plates

From: Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

Strain T2 T3 D-161646 C1 C2 C3
Lactose
 Mucic acid (g L−1) 8.0 ± 0.2 aA 7.7 ± 0.0 aB 5.4 ± 0.2 bA    
 Yield mucic acid/d-galacturonic acid (g g−1) 1.20 ± 0.05 a 1.13 ± 0.00 a 0.88 ± 0.05b    
d-Xylose
 Mucic acid (g L−1) 7.9 ± 0.3aA 7.8 ± 0.2aB 4.1 ± 0.1bB 3.4 ± 0.2aA 2.3 ± 0.1bA 1.0 ± 0.0cA
 Yield mucic acid/d-galacturonic acid (g g−1) 1.28 ± 0.05a 1.29 ± 0.08a 1.11 ± 0.18a 0.50 ± 0.03a 0.37 ± 0.01b 0.14 ± 0.01c
Glycerol
 Mucic acid (g L−1) 6.4 ± 0.2aB 5.3 ± 0.3bA 4.6 ± 0.1cB 4.5 ± 0.2aB 2.3 ± 0.1bA 1.2 ± 0.6cA
 Yield mucic acid/d-galacturonic acid (g g−1) 1.23 ± 0.02a 0.98 ± 0.04b 0.63 ± 0.02c 0.57 ± 0.02a 0.29 ± 0.02b 0.16 ± 0.01c
d-Glucose
 Mucic acid (g L−1) 6.5 ± 0.1aB 6.6 ± 1.1aAB 2.8 ± 0.2bC 5.1 ± 0.4aB 2.5 ± 0.2bA 1.1 ± 0.1cA
 Yield mucic acid/d-galacturonic acid (g g−1) 1.19 ± 0.01a 1.24 ± 0.05a 0.75 ± 0.09b 0.82 ± 0.05a 0.42 ± 0.02b 0.17 ± 0.01c
  1. Marine Trichoderma sp. LF328 transformants (T2 and T3) and Coniochaeta sp. MF729 transformants (C1, C2 and C3) were grown in 24-well plates with d-galacturonic acid as substrate and lactose, d-xylose, glycerol or d-glucose as co-substrate. T. reesei Δgar1 udh strain D-161646 was used as a positive control. Values are mean ± standard error of the mean for n = 3 biological replicates. Values for the same specie in the same row with the same superscript letter (a to c) did not differ significantly (p > 0.05). Values for mucic acid concentration in the same column with the same superscript letter (A to C) did not differ significantly (p > 0.05)