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Table 1 Mucic acid production characteristics of marine fungi in 24-well plates

From: Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

Strain

T2

T3

D-161646

C1

C2

C3

Lactose

 Mucic acid (g L−1)

8.0 ± 0.2 aA

7.7 ± 0.0 aB

5.4 ± 0.2 bA

   

 Yield mucic acid/d-galacturonic acid (g g−1)

1.20 ± 0.05 a

1.13 ± 0.00 a

0.88 ± 0.05b

   

d-Xylose

 Mucic acid (g L−1)

7.9 ± 0.3aA

7.8 ± 0.2aB

4.1 ± 0.1bB

3.4 ± 0.2aA

2.3 ± 0.1bA

1.0 ± 0.0cA

 Yield mucic acid/d-galacturonic acid (g g−1)

1.28 ± 0.05a

1.29 ± 0.08a

1.11 ± 0.18a

0.50 ± 0.03a

0.37 ± 0.01b

0.14 ± 0.01c

Glycerol

 Mucic acid (g L−1)

6.4 ± 0.2aB

5.3 ± 0.3bA

4.6 ± 0.1cB

4.5 ± 0.2aB

2.3 ± 0.1bA

1.2 ± 0.6cA

 Yield mucic acid/d-galacturonic acid (g g−1)

1.23 ± 0.02a

0.98 ± 0.04b

0.63 ± 0.02c

0.57 ± 0.02a

0.29 ± 0.02b

0.16 ± 0.01c

d-Glucose

 Mucic acid (g L−1)

6.5 ± 0.1aB

6.6 ± 1.1aAB

2.8 ± 0.2bC

5.1 ± 0.4aB

2.5 ± 0.2bA

1.1 ± 0.1cA

 Yield mucic acid/d-galacturonic acid (g g−1)

1.19 ± 0.01a

1.24 ± 0.05a

0.75 ± 0.09b

0.82 ± 0.05a

0.42 ± 0.02b

0.17 ± 0.01c

  1. Marine Trichoderma sp. LF328 transformants (T2 and T3) and Coniochaeta sp. MF729 transformants (C1, C2 and C3) were grown in 24-well plates with d-galacturonic acid as substrate and lactose, d-xylose, glycerol or d-glucose as co-substrate. T. reesei Δgar1 udh strain D-161646 was used as a positive control. Values are mean ± standard error of the mean for n = 3 biological replicates. Values for the same specie in the same row with the same superscript letter (a to c) did not differ significantly (p > 0.05). Values for mucic acid concentration in the same column with the same superscript letter (A to C) did not differ significantly (p > 0.05)