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Table 1 Proteins with native signal peptides used in this study

From: Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus

Product

Native organism

Protein

Signal peptide

Accession number

Temperature optimuma (°C)

SignalP-5.0 (probability)

PrediSI (score)

Cleavage sitec (amino acid)

α-Amylase

   

Sec/SPI

  

 amyL

B. licheniformis

BL00499

90

0.9792

0.9872

28/29, 28

 amyE

B. subtilis

BSU03040

50

0.9718

0.6027

29, 33/34

 amyS

G. stearothermophilus

GT50_07800

65

0.9807

1.0000

34/35, 34

 amy

B. methanolicus

BMMGA3_04345

ND

0.9931

0.6850

23/24, 24

Protease

 apr

B. licheniformis

BLI_RS05490

50

0.9935

0.9128

29/30, 29

 aprE

B. subtilis

GP2222_11200

60b

0.9929

0.8976

29/30, 29

 apr

G. stearothermophilus

GT50_00190

NC

0.6871

0.6283

30/31, 30

  1. Signal peptides were analyzed by SignalP-5.0 and PrediSI [69, 71]
  2. ND not determined, NC not characterized. To the best of the authors’ knowledge
  3. a Temperature optimum of proteins previously determined. See text for details and references
  4. b Temperature optimum for homolog protease-gene in B. subtilis A26
  5. c As predicted by SignalP-5.0 and PrediSI, respectively [69, 71]