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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus

Fig. 5

Mean fluorescence intensity (sfGFP) in supernatants of B. methanolicus recombinant strains carrying sfGFP gene. The sfGFP gene is fused to signal peptides from α-amylases derived either from Gstearothermophilus (spGs-sfGFP), B. subtilis (spBs-sfGFP), B licheniformis (spBl-sfGFP) or B. methanolicus (spBm-sfGFP). Fluorescence from strain carrying vector pBV2xp-sfGFP with no signal peptide (sp0-sfGFP) is used as control. Fluorescence from both uninduced (black bars) and induced (grey bars) cultures are indicated

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