Fig. 4From: Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicusMean fluorescence intensity (sfGFP) in pellet of B. methanolicus recombinant strains carrying sfGFP gene. The sfGFP gene is fused to signal peptides from either G. stearothermophilus (strain spGs-sfGFP), B. subtilis (strain spBs-sfGFP), B. licheniformis (strain spBl-sfGFP) or B. methanolicus (strain spBm-sfGFP). Fluorescence from strain carrying vector pBV2xp-sfGFP with no signal peptide (sp0-sfGFP) is used as control. Fluorescence from both uninduced (black bars) and induced (grey bars) cultures are indicatedBack to article page