Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus

Fig. 1

Detection of α-amylase activity from recombinant strains of B. methanolicus incubated at 50 °C. In the plate assay, hydrolysis of starch by α-amylase is seen as clearing zones around the colonies, visualized by addition of Lugol’s solution after 24 h incubation. Recombinant strains tested are: Plate a: EV (emtpy vector), used as control (A), spBl-amyBl (B), spBs-amyBs (C), spBm-amyBm (D) and spGs-amyGs (E). Plate b: Control strain EV (F), spGs-amyGs (G), spGs-amyBl (H) and spGs-amyBs (I)

Back to article page