A novel knock out strategy to enhance recombinant protein expression in Escherichia coli

Table 2 Comparative gain (%) in the protein expression in best performing single gene mutants and double gene mutants in comparison to the control strain

Top single gene mutants	Gain % wrt to control (BW25113)	Top double gene mutants	Gain % wrt to control (BW25113)
∆yacH	137.53	∆elaA + ∆cysW	315.01
∆marR	147.45	∆elaA + ∆cueR	279.68
∆eleA	239.10	∆cysW + ∆marR	267.85
∆cysJ	185.37	∆cysW + ∆yfbN	264.64
∆yhbC	159.71	∆cysW + ∆purL	263.82
∆purL	210.13	∆elaA + ∆yacH	247.40
∆cysW	268.06	∆cysJ + ∆yabI	247.33
∆cueR	227.85	∆elaA + ∆yhbC	238.01
∆yabI	156.90	∆eleA + ∆yacH	235.83
∆yfbN	258.43	∆cysJ + ∆yhbC	216.96

ISSN: 1475-2859