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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae

Fig. 5

Testing of selected designs to construct xylose repressive biosensors. a Characterization of the transcriptional activity of strain containing “LEU2p·1*xylO”, “1*xylOLEU2p” and “4*xylOLEU2p” with or without XylR-Med2. b Dose–response curves of the strain containing “LEU2p·1*xylO”, “1*xylOLEU2p” and “4*xylOLEU2p” with XylR-Med2 in the presence of xylose. c Characterization of the transcriptional activity of the strain containing “LEU2p·1*xylO”, “1*xylOLEU2p” and “4*xylOLEU2p” with or without Med2-XylR. d Dose–response curves of the strain containing “LEU2p·1*xylO”, “1*xylOLEU2p” and “4*xylOLEU2p” with Med2-XylR in the presence of xylose. Minimal medium containing 2% maltose was used as the carbon source in these tests. The data are presented as the means ± standard deviations from three independent experiments

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