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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae

Fig. 4

Testing selected designs to construct a fatty acyl-CoA repressive biosensor. a Characterization of the transcriptional activity of FadR-VPR and FadR-Med2 in the fatty acyl-CoA biosensor. b Characterization of the transcriptional activity of strains containing LEU2p·1*fadO, 1*fadOLEU2p, or 4*fadOLEU2p with FadR-Med2. c Dose–response curve of the strain containing LEU2p·1*fadO and FadR-Med2 in the presence of oleic acid (C18:1). The data are presented as the means ± standard deviations from three independent experiments

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