Fig. 3

Effects of the position and number of operators in different promoters. a Schematic design showing the positions and numbers of fapO site in the LEU2 promoter. b Different numbers and locations of the fapO site in the LEU2 promoter were evaluated. c Schematic design showing the positions and numbers of fapO site in the LEU2 core promoter and the synthetic minimal core promoter Core1. d Full-length LEU2 promoter was replaced with the LEU2 core promoter (LEU2cp) and the synthetic minimal core promoter (Core1p) and the effects were compared. e Dose–response curves of LEU2p·1*fapO, 1*fapOLEU2p, and 4*fapOLEU2p containing strains in the presence of different concentrations of cerulenin. The data are presented as the means ± standard deviations from three independent experiments