Fig. 6

Effect of an alternative translation start site within the coding sequence of SPEpr-Cut-11. a The original (wt) sequence of SPEpr-Cut-11 contains an ATG codon 9nt downstream of the annotated start codon which could act as alternative translation start site and would result in a Cut-11 variant shortened by the first three amino acids of the SPEpr. Both ATG codons were individually exchanged by ACG to force translation start from the remaining ATG. b Lipolytic activity and amount of extracellular Cut-11 (determined by split GFP assay) which was expressed from the original plasmids (wt) with varying sizes of spacers (4, 6 or 8nt) or with first (start 2) or second ATG (start 1) codon exchanged