Fig. 5From: The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilisIn silico analysis of translation initiation rates. Translation initiation rates (TIR, arbitrary units) for constructs with different spacer lengths were calculated using the RBS Calculator v2.0 [17, 18]. Mean values of experimentally determined changes of GFPmut3, GUS, Cut-11 and EXLX1-11 amounts (shown in Figs. 2 and 4) are indicatedBack to article page