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Fig. 4 | Microbial Cell Factories

Fig. 4

From: The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

Fig. 4

Influence of spacer length on the production of secreted proteins. X-fold change in Cut-11 activity and amount of Cut-11 and EXLX1-11 proteins determined by split GFP assay in culture supernatants of B. subtilis TEB1030 harboring expression plasmids pBSxnt-SPepr-cut-11/-EXLX1-11 (a/b), pBSxnt-SPpel-cut-11/-EXLX1-11 (c/d) and pBSxnt-SPbsn-cut-11/-EXLX1-11 (e/f) compared to the respective pBS4nt expression plasmid

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