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Fig. 2 | Microbial Cell Factories

Fig. 2

From: The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

Fig. 2

Determination of optimal spacer length for production of intracellular proteins GUS and GFPmut3. GUS activity and relative fluorescence units (RFU) for GFPmut3 were determined in cell extracts of B. subtilis TEB1030 harboring expression plasmids pBSxnt-GFPmut3, pBSxnt-GUS or the empty vector control pBSMul1 (ev) and calculated as x-fold changes compared to the basic constructs pBS4nt-GFPmut3 or -GUS which was arbitrarily set to 1

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Microbial Cell Factories

ISSN: 1475-2859

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