Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

Fig. 1

Nucleotide composition of ribosome binding sites of plasmids pBS4nt to pBS12nt depicting different spacer lengths. Shine Dalgarno (SD) sequence (red), spacer (green) and start of target gene (blue) are highlighted. The cleavage site for NdeI including the start codon (bold) is underlined. Spacer sequences were varied in length from 4 to 12nt by insertion of adenosines. The original plasmid pBSMul1 is designated here as pBS4nt to indicate the length of the spacer

Back to article page