Fig. 5
Chromatin immunoprecipitation (ChIP) analysis of the binding of SacR1 and SacR2 to the transcription factor binding sites (TFBSs) in the three promoter regions. a Detection of FLAG-tagged SacR1 and SacR2 proteins by western blotting with a FLAG-specific antibody. 1, SacR1; 2, SacR1. b Enrichment of FLAG-tagged SacR1 and SacR2 with TFBSs as determined by ChIP-qPCR. SacR1-Tpts1, SacR1-TsacA, SacR1-Tagl4, SacR1-TsacR2 represent the binding of SacR1 to the TFBSs and SacR2-Tpts1, SacR2-TsacA, SacR2-Tagl4, SacR2-TsacR2 represent the binding of SacR2 to the TFBSs. Data are presented as mean values based on at least three replicates. Error bars indicate standard deviations. Values that differed significantly (P < 0.05) from those of the negative control (normal rabbit IgG) are indicated with asterisks