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Fig. 4 | Microbial Cell Factories

Fig. 4

From: The local transcriptional regulators SacR1 and SacR2 act as repressors of fructooligosaccharides metabolism in Lactobacillus plantarum

Fig. 4

Characterization and verification of SacR1-DNA and SacR2-DNA binding at the four promoter regions by electrophoretic mobility shift assays (EMSAs). a, b The binding of His6-tagged SacR1 with DNA fragments of promoter region of Ppts1-sacA and its mutated site. c, d The binding of His6-tagged SacR2 with DNA fragments of promoter region of Pagl4 and its mutated site. e, f The binding of His6-tagged SacR2 with DNA fragments of promoter region of PsacR2 and its mutated site. The positions of the SacR1-DNA and SacR2-DNA complexes (c) or free DNA (f) are indicated at the left of the figure

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Microbial Cell Factories

ISSN: 1475-2859

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