Table 4 List of strains and plasmids used in this study

E. coli strain

 NEB10β

Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (Str^R) rph spoT1 Δ(mrr-hsdRMS-mcrBC)

New England Biolabs

 BL21(DE3)

E. coli str. B F⁻ ompT gal dcm lon hsdS_B(r⁻_B m⁻_B ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB⁺]_K-12(λ^S)

New England Biolabs

 eBL04

[BL21(DE3)] ΔtyrR pET28-pYIBN-aroG^(fbr)-B30rbs-tyrA^(fbr)-tRRNC; Kan^R

[28]

 eBL0400DT

[eBL04] pCDFDuet-1; Kan^R; Spc^R

[28]

 eBL0430T

[eBL04] pCDF-pLPP-B30rbs-TcXAL-T7t; Kan^R; Spc^R

This study

 eBL0432T

[eBL04] pCDF-pLPP-B32rbs-TcXAL-T7t; Kan^R; Spc^R

This study

S. cerevisiae strain

 BY4741

MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

ATCC

 sBY10

[BY4741] pTEF1-At4CL-tADH1-pPGK1-VvSTS-tCYC1 (integration with Kluyveromyces lactis LEU2 marker)

This study

 sBY11

[sBY10] pTEF1-ACC1^S659A,S1157A-tADH1 (integration with K. lactis LEU2 and URA3 markers)

This study

Plasmids

 pCDF-Duet-1

For construction p-coumaric acid producing plasmids

[28]

 pETM-TAL-4CL

For amplification of TcXAL gene

[30]

 pCfB1020

For construction resveratrol expression cassette

[10]

 pCfB1175

For construction ACC1 mutant expression cassette

[10]

 pCDF-pLPP-B30rbs-TcXAL-T7t

For p-coumaric acid production testing

This study

 pCDF-pLPP-B32rbs-TcXAL-T7t

For p-coumaric acid production testing

This study