Fig. 4
From: Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma
a Agarose gel-electrophoresis of RNA, isolated from purified hIFNγ IBs. 1—RNA, isolated from purified hIFNγ IBs; 2—total RNA, isolated from non-transformed E. coli cells. b, c Dot-blot hybridization of RNA isolated from hIFNγ IBs. b RNA was dotted on nitrocellulose filters, hybridized with 32P-labelled oligonucleotide specific for hIFNγ mRNA, striped and c reprobed with oligonucleotide specific for the E. coli 16S rRNA. Total amount of RNA on the dots: A1 and A2—10 µg; B1 and B2—5 µg; C1 and C2—2.5 µg; D1 and D2—1 µg; A3—10 µg RNA pre-treated with DNase; B3—10 µg total RNA isolated from transformed E. coli cells; C3 and D3—10 µg total RNA isolated from non-transformed E. coli cells