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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Expression, homology modeling and enzymatic characterization of a new β-mannanase belonging to glycoside hydrolase family 1 from Enterobacter aerogenes B19

Fig. 3

The prediction and analysis of the catalytic domin performed by structural superposition. a The surface view of Man1E docking mannotriose, red stick represented mannotriose. b The surface view of RmMan5B (PDB code, 4LYP) from Rhizomucor miehei docking mannotriose, red stick represented mannotriose. c Structural superposition of amino acid residues in the active domain of Man1E and RmMan5B-mannotriose, violet represented Man1E, cyan represented RmMan5B. d Amino acid residues in the catalytic module of the complex of Man1E–mannotriose, green represented the acid–base residue and the nucleophilic residue, yellow represented hydrophobic residues, cyan represented other residues. All figures were created by PyMOL 2.3.2. e Diagrammatic sketch of hydrogen bond interactions between mannotriose and amino acid residues at subsites − 1 to + 2. W1, W2, W3 represented water molecules. The figure were drown using ChemDraw software

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