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Table 2 Primers used in this study

From: Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars

Primer

Sequence

Mr-αGluF

ATGACTGACGCTATTTGGTGG

Mr-αGluR

CTAGTTCTTGACAATGTACAATCTACTCTC

RFpET28F

CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGACTGACGCTATTTGGTGGAAAG

RFpET28R

GATCTCAGTGGTGGTGGTGGTGGTGGTTCTTGACAATGTACAATCTACTCTCG

D206AF

TCAGAATCGCGACTGCTGGTATGTACTCCAAGGTGCAAACCTTTGAGG

D206AR

ACCAGCAGTCGCGATTCTGAAACCGTCCACACCCTTCTCGAACCAG

E264QF

ACTGTTGGTCAGGTGGGCCACTCTTCTCGTGAAGATGCCTTGAAGTACGTC

E264QR

GTGGCCCACCTGACCAACAGTCATGGCGTCGTACTTGGAGGTCACCTTC

D339AF

GAGAACCACGCGCAACCAAGATGTATCACTCGTTTCGGTAACGACAGCCCAG

D339AR

CATCTTGGTTGCGCGTGGTTCTCGAAGAAACAAGTTGACCATGCATCAGTTCC

  1. Sequences complementary to the insertion site in the pET28b(+) plasmid are underlined. Initial ATG and TAG stop codons are indicated in bold
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Microbial Cell Factories

ISSN: 1475-2859

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