Fig. 5From: Design and development of a chimeric vaccine candidate against zoonotic hepatitis E and foot-and-mouth diseaseAntigenicity of the HEV-FMDV chimeric proteins. a Western blot analysis using anti-HEV 5G5 monoclonal antibody (left), commercial porcine polyclonal antibodies used against FMD (middle) and serum of mice free of anti-HEV and anti-FMDV antibodies (right); M: molecular weight marker; lanes 1-4: Seq 8, Seq 8-P216, Seq 8-P222 and P222, respectively. b Assessment by indirect ELISA of the reactivity of the chimeric antigens against the anti-FMDV antibodies present in the sera of pigs infected with FMDV strains. c Assessment of non-specific reactivity of the chimeric antigens using mice sera free of anti-FMDV and anti-HEV antibodies. d Reactivity of the chimeric antigens against the HEV-neutralizing 5G5 monoclonal antibodyBack to article page