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Table 2 0.05 IPTG with 1 μ or 10 μ phage, proximate colony number

From: A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)

Strain

1 μ phage

10 μ phage

4 h

16 h

4 h

16 h

BL21(pT7cas9,prfp) without phage

6.65*108

2.79*109

7.7*108

1.285*109

BL21(pT7cas9, prfp) with phage

16

0

9

0

BL21(pT7cas9, p3gRNA, prfp)

4.8*107

2.6*106

2.7*107

1.011*105

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Microbial Cell Factories

ISSN: 1475-2859

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