Table 1 The plasmids used in this study
From: A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)
Plasmids | Characteristics | |
|---|---|---|
pCas9 | Cas9 with constitutive promoter, Cm | Lab stock (Jiang et al. 2013) |
pTFG025 | Arabinose operon, Cas9, Kmr | Lab stock |
pT7-Cas9 | Cas9 with T7 promoter, Amp | Lab stock |
pgRNA | Plasmid for gRNA expression | Lab stock |
pTgRNA | Derived from pgRNA targeting tail tubular protein B gene, Apr | This study |
pCgRNA | Derived from pgRNA targeting capsid assembly protein gene, Apr | This study |
p3.8gRNA | Derived from pgRNA targeting 3.8 protein gene, Apr | This study |
p3gRNA | Derived from pgRNA targeting tail tubular protein B, capsid assembly protein and 3.8 protein, Apr | This study |
pT7-TgRNA | Derived from pTgRNA, replacing the constitutive promoter by T7 promoter, Apr | This study |
pT7-CgRNA | Derived from pCgRNA, replacing the constitutive promoter by T7 promoter, Apr | This study |
pT7-3.8gRNA | Derived from p3.8gRNA, replacing the constitutive promoter by T7 promoter, Apr | This study |
pT7-3gRNA | Derived from p3gRNA, replacing the constitutive promoter by T7 promoter, Apr | This study |
pBBR1MCS2 | broad-range host vector used for conjugation, Kmr | lab stock |
prfp | derived from pBBR1MCS2, BBa_J23100-rfp, Kmr | Lab stock |