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Fig. 3 | Microbial Cell Factories

Fig. 3

From: A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)

Fig. 3

Growth curves of BL21(DE3) strains carrying the optimized CRISPR/Cas9 system with T7 phage infection. a Growth curves of strains based on the pBad promoter. Cultures were infected with 1 μl of phage stock in the presence 2 g/L arabinose. b Growth curves of strains based on the T7 promoter infected with 1 μl of phage stock with 0.4 mM IPTG. c Growth curves of strains based on the T7 promoter with 0.05 mM IPTG. d Growth curves of strains based on the T7 promoter infected with 10 μ of phage stock with 0.05 mM IPTG. e T7 based strains infected with 1 μl of phage stock with 0.05 mM IPTG

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