An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Table 6 Sequence coverage obtained for NHSSP at different pH conditions with no enzyme specificity

Condition	Sequence coverage^a			Average seq. coverage (%)	rSD (%)
Condition	Replicate 1 (%)	Replicate 2 (%)	Replicate 3 (%)	Average seq. coverage (%)	rSD (%)
pH 6.5	72	81	97	83	12.4
pH 6.0	82	85	82	83	1.7
pH 5.0	86	70	76	77	8.5

ISSN: 1475-2859