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Table 5 Sequence coverage obtained for NHSSP at different pH conditions

From: An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Condition

Sequence coveragea

Average seq. coverage (%)

rSD (%)

Replicate 1 (%)

Replicate 2 (%)

Replicate 3 (%)

pH 6.5

6

6

11

8

30.7

pH 6.0

6

1

7

8

22.2

pH 5.0

6

3

3

4

35.4

  1. aCleavage specificity after F, Y and W, reference protein-BSA
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Microbial Cell Factories

ISSN: 1475-2859

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