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Table 4 Number of residues in test proteins (BLG and mAb) exhibiting NHSSP cleavage

From: An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Residue

BLG

mAb heavy chain

mAb light chain

# residues

% of possible residues (%)

# residues

% of possible residues (%)

# residues

% of possible residues (%)

A

5

33.3

1

4.2

1

6.7

C*

1

20.0

0

0.0

0

0.0

D

0

0.0

2

11.8

0

0.0

E

1

6.3

1

5.0

2

18.2

F

3

75.0

3

23.1

4

57.1

G

0

0.0

4

12.9

2

15.4

H

0

0.0

2

20.0

0

0.0

I

0

0.0

0

0.0

0

0.0

K

2

13.3

8

22.2

3

23.1

L

12

54.5

8

25.0

4

30.8

M

2

50.0

0

0.0

1

100

N

0

0.0

6

31.6

2

28.6

P

0

0.0

0

0.0

0

0.0

Q

5

55.6

4

22.2

4

33.3

R

1

33.3

0

0.0

1

16.7

S

2

28.6

7

13.2

7

20.0

T

0

0.0

3

7.9

3

17.6

V

1

11.1

3

7.0

1

6.3

W

0

0.0

1

11.1

1

25.0

Y

2

50.0

6

27.3

6

66.7

  1. The aa that were found with a certain preference are in italics
  2. If the minimum aa residue number was not reached, they are underlined