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Table 3 Sequence coverage obtained with different proteins and enzymes

From: An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Enzyme

Sequence coverage (nanoLC-ESI–MS/MS analytics)a

BSA (%)

BLG (%)

mAb HC (%)

mAb LC (%)

Trypsin

93

76

64

71

NHSSP

91

100

84

96

  1. aSpecificity R, K for trypsin and no specificity for NHSSP; ion cut-off 20
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Microbial Cell Factories

ISSN: 1475-2859

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