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Fig. 3 | Microbial Cell Factories

Fig. 3

From: An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Fig. 3

Purified NHSSP variants and SDS effect. a Purified del-NHSSP expressed by pPink-HC-del-NHSSP clone. Lane M, Amersham™ LMW Calibration Kit For SDS Electrophoresis; lane 1, NHSSP sample denatured by heating in Leammli electrophoresis loading buffer (with SDS); lane 2, NHSSP sample denatured by heating in Leammli electrophoresis loading buffer (with SDS) in the presence of 5 mM PMSF. b Very long electrophoresis run of purified NHSSP and del-NHSSP. Lane M, Amersham™ LMW Calibration Kit For SDS Electrophoresis; lane 1, NHSSP expressed by pPink-HC-NHSSP clone; lane 2, del-NHSSP expressed by pPink-HC-del-NHSSP clone

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Microbial Cell Factories

ISSN: 1475-2859

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