Primer | Sequence (5′→3′) |
---|---|
For construction and verification | |
 SNF1-F | GGAATTCCAGATCTCCTCGAGATGAGCAGTAACAACAACACAAACA |
 SNF1-R | ATCTATCGCAGATCCCTCGAGTCAATTGCTTTGACTGTTAACGG |
 SIP1-F | GGAATTCCAGATCTCCTCGAGATGGGAAACAGTCCTTCTACTCAGGAT |
 SIP1-R | ATCTATCGCAGATCCCTCGAGCTAATTACTGATCTGAGACTTTTGTG |
 SIP2-F | GGAATTCCAGATCTCCTCGAGATGGGTACTACGACAAGTCATCCAG |
 SIP2-R | ATCTATCGCAGATCCCTCGAGTTACGAGGACTCTATGGGCGTATAAAG |
 GAL83-F | GGAATTCCAGATCTCCTCGAGATCTATCGCAGATCCCTCGAG |
 GAL83-R | ATCTATCGCAGATCCCTCGAGCTATTGCAATGGTGTATACAGTATTTGG |
 Kan-F | AGAGTCGACCTGCATGCCAGCTGAAGCTTCGTACGCTG |
 Kan-R | GCCAGTGCCAAGCTTGCATGCGCATAGGCCACTAGTGGATCTGA |
 PGK-F | TCTAACTGATCTATCCAAAACTGA |
 PGK-R | TAACGAACGCAGAATTTTC |
 K-F | CAGCTGAAGCTTCGTACGC |
 K-R | GCATAGGCCACTAGTGGATCTG |
For real-time PCR | |
 HXT1-F | TGTGCCATTGGTGGTATCGT |
 HXT1-R | ACCACCGACACCTAAACCAG |
 HXK1-F | TACTGGTGTCAACGGTGCTT |
 HXK1-R | GTTCGTCGACAGCAACATCG |
 GLK1-F | TCTGCGACGATTTCGAGGTT |
 GLK1-R | CTTTGTCCGAGGCCAATGTG |
 PFK1-F | CAGAAAGCGTGAGGGTCGTA |
 PFK1-R | TCGATGGAACCGACAAGACC |
 PFK2-F | CGCAGTTTCAACCAAGCCAA |
 PFK2-R | AAGATAGCGGAACGCACGAT |
 TDH1-F | TTGAGGTTGTTGCTGTCAACG |
 TDH1-R | GCTTGTCGTCATGGGAAACAG |
 ACT1-F | ACGCTCCTCGTGCTGTCTTC |
 ACT1-R | GTTCTTCTGGGGCAACTCTCA |