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Table 2 Oligonucleotide primers used in this study

From: Identification and characterization of a moonlighting protein-enolase for surface display in Streptococcus thermophilus

Primer

Sequence (5ʹ–3ʹ)

Restriction site(s)

gfpF

GGAATTCCATATGAGCAAAGGAGAAGAACTTTTCA

NdeI

gfpR1

CCCAAGCTTGTAGAGCTCATCCATGCCATGTGTA

HindIII

gfpR2

GTAGAGCTCATCCATGCCATGTGTA

 

gfp-enoF

ATGGCATGGATGAGCTCTACATGTCAATTATTACTGATGTCTA

 

enoR1

CCCAAGCTTTTATTTTTTCAAGTTGTAGAATGAT

HindIII

galF1

CATGCCATGAACATGACTGAAAAAATTCAAA

NcoI

galR2

CCGCTCGAGATTTAGTGGTTCAATCATGAAGCTT

XhoI

galF2

CGGGATCCAATGAACATGACTGAAAAAATTCAAA

BamHI

galR2

ATTTAGTGGTTCAATCATGAAGCTT

 

gal-enoF

TCATGATTGAACCACTAAATATGTCAATTATTACTGATGT

 

enoR2

ACGCGTCGACTTATTTTTTCAAGTTGTAGAATGAT

SalI