Fig. 2
1H-13C HSQC NMR spectra of the reaction mixture of transglycosylation catalyzed by the native BGL-1 on pNPG as substrate (3 mM) in the presence of 13C-labelled glucose. a Detail of the region of the spectra over 13C 75 ppm. The anomeric region is presented for only the signals of labelled glucose, either unreacted or as part of the transglycosylation product. The regiochemistry corresponding to substitution on positon 2 on the labeled glucose is deduced from the superimposition of the spectra of the reaction mixture (in blue) with the spectra of: b sophorose (in red). The signals from H1′ of α- and β-sophorose are not observed in the spectrum of the reaction mixture (blue) since the non-reducing residue comes from the unlabeled donor; c cellobiose (in red); d laminaribiose (in red)