Fig. 5From: Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoterPurification of thermostable β-galactosidase and pisGDH expressed in Thermus cells. a Purification of thermostable β-galactosidase. b Purification of pisGDH. (−); 0 mM silica, (+); 10 mM silica. Lane P indicates the fraction purified via nickel-affinity chromatography on a Ni-NTA column. Lane M contains the molecular mass markerBack to article page