Table 1 Key genetic changes in evolved strains
Locus_Tag | Gene | Product | Reference sequence location | Mutation |
|---|---|---|---|---|
MEALZ_RS01065 | hppA | Sodium-translocating pyrophosphatase | 223,987 | N30S (AAC→AGC) |
MEALZ_RS01220 | cysB | LysR family transcriptional regulator | 264,570 | E154D (GAA→GAC) |
MEALZ_RS01235 | cysA | Sulfate ABC transporter ATP-binding protein | 268,089 268,091 268,121 | K19D (AAA→GAC) K19D (AAA→GAC) S9G (AGC→GGC) |
MEALZ_RS01240 | cysW | Sulfate ABC transporter permease subunit CysW | 269,090 269,198 269,207 | S63A (TCT→GCT) L27M (CTG→ATG) R24C (CGC→TGC) |
MEALZ_RS01250 | sbp | Sulfate ABC transporter substrate-binding protein | 270,878 | K267N (AAA→AAT) |
271,623 | A19V (GCT→GTT) | |||
MEALZ_RS01295 | fabG | 3-Oxoacyl-ACP reductase FabG | 282,479 282,486 | Y78H (TAC→CAC) A80V (GCT→GTT) |
MEALZ_RS06915 | folD | Bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD | 1,598,870 | D87E (GAT→GAA) |
MEALZ_RS16400 | rpoC | DNA-directed RNA polymerase subunit beta | 3,871,938 | D170Y (GAT→TAT) |
MEALZ_RS17985 |  | Outer membrane lipoprotein carrier protein LolA | 4,233,780 | A183V (GCC→GTC) |
MEALZ_RS18010 |  | Transcriptional regulator | 4,241,883 | S389G (AGT→GGT) |