Fig. 3
Adaptive evolution approach used to isolate evolved strains and characterization of evolved strains by NGS-based studies (a). The central metabolism of Δpfk ALE strain grown on methane. Genes highlighted in black were significantly up-regulated in Δpfk ALE strain compared to unevolved strain (b), Clusters of Orthologous Groups (COGs) categories of up-regulated and down-regulated genes in pfk ALE strain compared to unevolved strain (c)