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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Saccharomyces cerevisiae nutrient signaling pathways show an unexpected early activation pattern during winemaking

Fig. 7

Direct comparison made between the laboratory and winemaking conditions. a Snf1 phosphorylation was measured by a Western blot analysis. The samples from strains EC1118 and BQS252 under laboratory conditions of derepression at a low glucose concentration, as described in Fig. 3a, were run together with the selected samples (8 h and days 1 and 2) under winemaking conditions (Fig. 5a). A specific antibody against the Snf1 phosphorylated form was used. An anti-polyHis antibody was utilized to detect total Snf1. b The Gln3 phosphorylation Western blot analysis. The samples from strains EC1118 and BQS252 under laboratory conditions with the addition of MSX and rapamycin, as described in Fig. 3b, were run together with the selected samples (8 h and days 1 and 2) under winemaking conditions (Fig. 5a). The tagged version of Gln3 was detected with an anti-Myc antibody

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