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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Optimization of hydrogenobyrinic acid biosynthesis in Escherichia coli using multi-level metabolic engineering strategies

Fig. 2

Optimization of the relative expression ratios of ALAS, PBGS, PBGD and UROS using RBS libraries. a Colonies were randomly selected and analyzed by PCR to determine the successful assembly of p15ASI-hemABCD. M marker, 23 colonies were selected from an LB plate. b The results of 96-deep-well plate fermentation of 288 positive strains. The Y axis represents the fluorescence intensity of the fermentation broth excited at 378 nm, and the strains are arranged according to the fluorescence intensity from low to high. c Evaluation of the HBA production capacity of five strains with the highest fluorescence value. Samples of the fermentation broth for HBA analysis were taken at 20 h after induction. Error bars indicate standard deviations from three biological replicates

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