Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Obtaining a series of native gradient promoter-5′-UTR sequences in Corynebacterium glutamicum ATCC 13032

Fig. 2

Construction of the PUTR library. With the backbone plasmid pXK99E-mCherry(m) as the starting plasmid, plasmids with different PUTRs were constructed. a Backbone plasmid; b Linearization of backbone plasmid by endonucleases (XbaI, SalI). PUTR fragments were amplified from C. glutamicum ATCC 13032 genome; c Linking of linearized backbone plasmid to PUTR fragments

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us