Table 3 Strains and plasmids used in this study
Strain/plasmid | Description | Sources or reference |
|---|---|---|
E. coli | ||
 JM109 | recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1 | Laboratory strain |
C. glutamicum | ||
 SSAAI | C. glutamicum SYPS-33a with deletion of the 591 bp in the C-terminus of serA, deletion of sdaA,alaT,avta and attenuation of ilvBN | [5] |
 A36 | SSAAI mutant strain | [23] |
 SSAAI-thrE | SSAAI harboring plasmid pDXW-10-thrE | This study |
 SSAAIΔthrE | SSAAI with deletion of thrE | This study |
 SSAAIΔNCgl2050 | SSAAI with deletion of NCgl2050 | This study |
 SSAAIΔNCgl2065 | SSAAI with deletion of NCgl2065 | This study |
 SSAAIΔNCgl0580 | SSAAI with deletion of NCgl0580 | This study |
 SSAAI-10 | SSAAI harboring plasmid pDXW-10 | This study |
 SSAAI-egfp | SSAAI harboring plasmid pDXW-10-egfp | This study |
 SSAAI-serE-egfp | SSAAI harboring plasmid pDXW-10-serE-egfp | This study |
 SSAAI-NCgl0581 | SSAAI harboring plasmid pDXW-10- NCgl0581 | This study |
 SSAAI-NCgl0581-serE | SSAAI harboring plasmid pDXW-10- NCgl0581-serE | This study |
 SSAAIΔNCgl0581 | SSAAI with deletion of NCgl0581 | This study |
 SSAAIΔNCgl0581-1 | SSAAIΔNCgl0581 harboring pDXW-11-1 | This study |
 SSAAIΔNCgl0581-0 | SSAAIΔNCgl0581 harboring pDXW-11-0 | This study |
 SSAAIΔNCgl0580- NCgl0580 | SSAAIΔserE harboring plasmid pDXW-10-serE (NCgl0580) | This study |
 SSAAI-serE | SSAAI harboring plasmid pDXW-10-serE (NCgl0580) | This study |
 ATCC13032 | Wild type | Laboratory strain |
 ATCC13032ΔserE | ATCC13032 with deletion of serE (NCgl0580) | This study |
 pK18mobsacB | Integration vector, oriV, oriT, mob, sacB, Kmr | [37] |
 pK18mobsacBΔthrE | pK18mobsacB carrying the up- and downstream homologous fragments of thrE gene for thrE deletion | This study |
 pK18mobsacBΔNCgl2050 | pK18mobsacB carrying the up- and downstream homologous fragments of NCgl2050 gene for NCgl2050 deletion | This study |
 pK18mobsacBΔNCgl2065 | pK18mobsacB carrying the up- and downstream homologous fragments of NCgl2065 gene for NCgl2065 deletion | This study |
 pK18mobsacBΔNCgl0580 | pK18mobsacB carrying the up- and downstream homologous fragments of NCgl0580 gene for NCgl0580 deletion | This study |
 pK18mobsacBΔNCgl0581 | pK18mobsacB carrying the up- and downstream homologous fragments of NCgl0581 gene for NCgl0581 deletion | This study |
 pDXW-10 | E. coli-C. glutamicum shuttle vector, tacM promoter, Kmr | [38] |
 pDXW-10-thrE | pDXW-10 carrying the gene of thrE | This study |
 pDXW-10-serE | pDXW-10 carrying the gene of serE | This study |
 pDXW-10-egfp | pDXW-10 carrying the gene of egfp | This study |
 pDXW-10-egfp-serE | pDXW-10 carrying the gene of egfp and serE for the expression of fusion protein EGFP-SerE | This study |
 pDXW-10- NCgl0581 | pDXW-10 carrying the gene of NCgl0581 | This study |
 pDXW-10- NCgl0581-serE | pDXW-10 carrying the gene of NCgl0581 and serE | This study |
 pDXW-11 | E. coli-C. glutamicum shuttle vector, probe plasmid, Kmr | [39] |
 pDXW-11-1 | pDXW-11 carrying the fragments of NCgl0581, the intergenic region between NCgl0581 and NCgl0580, and egfp | This study |
 pDXW-11-0 | pDXW-11 carrying the fragments of the intergenic region between NCgl0581 and NCgl0580, and egfp | This study |